Examples of transposon insertion distribution into genes that express proteins with essential and non-essential domains. Panel A shows two examples of genes with multiple protein domains, one of which is essential and the other is not. In panel B, two non-essential genes, oxyR and recQI, are shown. Of note is the lack of transposon insertions into the latter regions of these genes. This would not be predicted based on the prevalence of TA sites in the non-inserted region of oxyR or recQI. GenomeView (http://genomeview.org/ webcite) was utilized for mapping and visualization of the transposon mutant insertion sequencing reads from Illumina sequencing to the P. gingivalis base genomes. BED file tracks created from Galaxy platform analyses were used for mapping insertions to a GenBank file base track.
Klein et al. BMC Genomics 2012 13:578 doi:10.1186/1471-2164-13-578