Number of differentially detected genes between the preparation methods for each cell line. A: Genes detected at a significantly higher level (A), lower level (B) or with no difference (C) in total RNA compare to cytoplasmic RNA. The percentages of differentially detected genes were: A-431 18%, U-251MG 15%, and U-2 OS 6%; calculated as the sum of genes at a higher and lower level divided by the total number of detected genes.
Solnestam et al. BMC Genomics 2012 13:574 doi:10.1186/1471-2164-13-574