A global-view of the time-course profiles of reverse regulators of T helper cell differentiation and the time-course profiles of differentially regulated genes. (A) The set of genes that are specific in Th0, Th1, and Th2 are clustered in two clusters. The lower cluster holds altogether 34 unfiltered genes and the upper cluster contains only six genes. Most of the Th0, Th1 and Th2 specific genes are preferentially expressed in Th1 cells and have a lower expression level in Th2 cells. (B) The kinetics of the genes LIGAP identified to be differentially regulated are clustered in five clusters. Information about differential regulation is shown with colored dots. Consistent with (A), majority of the 34 genes specific in Th0, Th1, and Th2 are assigned to the fifth cluster, whereas the six genes are assigned to the third cluster. (A and B) Clusters are indicated with different colors on the branches in the dendrogram and with horizontal white lines in the heat maps. Standardized expression values are shown. The probe set data was standardized across the time points and lineages.
Äijö et al. BMC Genomics 2012 13:572 doi:10.1186/1471-2164-13-572