Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Research article

The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential

Lucas P Parizzi12, Maria Carolina B Grassi12, Luige A Llerena1, Marcelo F Carazzolle1, Verônica L Queiroz2, Inês Lunardi2, Ane F Zeidler2, Paulo JPL Teixeira1, Piotr Mieczkowski3, Johana Rincones2 and Gonçalo AG Pereira1*

Author affiliations

1 Laboratório de Genômica e Expressão, Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, Campinas, 13083-970, São Paulo, Brazil

2 Braskem S.A, CP 6192, Campinas, 13083-970, São Paulo, Brazil

3 Department of Genetics, School of Medicine, Carolina Center for Genome Sciences, University of North Carolina, Wilson Hall, Rm 341, CB#3280, Chapel Hill, NC, 27599-3280, USA

For all author emails, please log on.

Citation and License

BMC Genomics 2012, 13:562  doi:10.1186/1471-2164-13-562

Published: 19 October 2012

Abstract

Background

Synthetic biology allows the development of new biochemical pathways for the production of chemicals from renewable sources. One major challenge is the identification of suitable microorganisms to hold these pathways with sufficient robustness and high yield. In this work we analyzed the genome of the propionic acid producer Actinobacteria Propionibacterium acidipropionici (ATCC 4875).

Results

The assembled P. acidipropionici genome has 3,656,170 base pairs (bp) with 68.8% G + C content and a low-copy plasmid of 6,868 bp. We identified 3,336 protein coding genes, approximately 1000 more than P. freudenreichii and P. acnes, with an increase in the number of genes putatively involved in maintenance of genome integrity, as well as the presence of an invertase and genes putatively involved in carbon catabolite repression. In addition, we made an experimental confirmation of the ability of P. acidipropionici to fix CO2, but no phosphoenolpyruvate carboxylase coding gene was found in the genome. Instead, we identified the pyruvate carboxylase gene and confirmed the presence of the corresponding enzyme in proteome analysis as a potential candidate for this activity. Similarly, the phosphate acetyltransferase and acetate kinase genes, which are considered responsible for acetate formation, were not present in the genome. In P. acidipropionici, a similar function seems to be performed by an ADP forming acetate-CoA ligase gene and its corresponding enzyme was confirmed in the proteome analysis.

Conclusions

Our data shows that P. acidipropionici has several of the desired features that are required to become a platform for the production of chemical commodities: multiple pathways for efficient feedstock utilization, ability to fix CO2, robustness, and efficient production of propionic acid, a potential precursor for valuable 3-carbon compounds.

Keywords:
Genome; Propionibacterium acidipropionici; Propionic acid; Biotechnology