Additional file 3.

Figure S2. Bioinformatic evidence for a role of E2F1 transcription factor in contributing to miRNA-associated expression profiles. Panel A graphically represents the Transcription factor association of canonically modulated genes subsequent miR-181b over-expression or inhibition in HEK-293, HeLa, and SH-SY5Y cell models. The TRANSFAC function of GATHER was used to identify significantly enriched transcription factor signatures within modulated genes. A Bayes factor of 6 was used for threshold significance, which in each case corresponded to p<0.0001. Panel B illustrates predicted binding sites for schizophrenia-associated miR-181b, miR-107, and miR-20a in the 3′-UTR of E2F1, as well as the AU-rich element in this 3′-UTR. These predicted binding sites were identified using the miRanda shell algorithm; only sites with threshold scores greater than 120 are shown in this figure.

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Carroll et al. BMC Genomics 2012 13:561   doi:10.1186/1471-2164-13-561