miRNA-mediated regulation of E2F1 3′-UTR reporter gene expression. The sensitivity of the E2F1 3′-UTR to intracellular 181b, miR-107, and miR-20a levels was determined by luciferase reporter gene expression in the presence of either synthetic miRNA or corresponding anti-miR inhibitor. In each case the response was normalised against the respective miRNA and anti-miR control oligos. This data was obtained from n=4 experiments, each performed in triplicate.
Carroll et al. BMC Genomics 2012 13:561 doi:10.1186/1471-2164-13-561