Reporter gene analysis of miRNA recognition elements (MRE). Putative MREs from genes modulated by miRNA expression were cloned into the 3′-UTR of the firefly luciferase gene in pMIR-REPORT. Responsiveness of the firefly luciferase reporter gene to increased miR-181b expression (miR-181b transfection) was analysed with respect to a pRL-TK renilla luciferase control. Data was normalised against mutant miR-181b miRNA control transfection. This data was obtained from n=4 experiments, each performed in triplicate, and analysed using a one-tailed T-test.
Carroll et al. BMC Genomics 2012 13:561 doi:10.1186/1471-2164-13-561