Figure 6.

Reporter gene analysis of miRNA recognition elements (MRE). Putative MREs from genes modulated by miRNA expression were cloned into the 3-UTR of the firefly luciferase gene in pMIR-REPORT. Responsiveness of the firefly luciferase reporter gene to increased miR-181b expression (miR-181b transfection) was analysed with respect to a pRL-TK renilla luciferase control. Data was normalised against mutant miR-181b miRNA control transfection. This data was obtained from n=4 experiments, each performed in triplicate, and analysed using a one-tailed T-test.

Carroll et al. BMC Genomics 2012 13:561   doi:10.1186/1471-2164-13-561
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