The performance of conserved and non-conserved target predictions across multiple biological datasets. Panel A illustrates the accuracy with which modulated genes were correctly predicted as either targets or non-targets by Targetscan. Error bars were calculated using the mean, N, and standard deviation across HEK-293, HeLa, and SH-SY5Y datasets. Panel B illustrates the false discovery rates associated with Targetscan’s prediction of genes modulated subsequent to altered miRNA expression. A false negative indicates a gene differentially expressed with miRNA modulation, but not a predicted miR-181b target; and a false positive indicates a predicted miR-181b target that is not differentially expressed with miRNA modulation. Error bars were calculated using the mean, N, and standard deviation across HEK-293, HeLa, and SH-SY5Y datasets. Panel C shows the conservation status of predicted target genes modulated in response to altered miR-181b expression. The values in this figure represent the average values across both miR-181b over-expression and inhibition in HEK-293, HeLa, and SH-SY5Y cell types. PCT: Probability of conserved targeting; the lower the probabilistic value, the poorer the conservation of the predicted binding site across multiple species.
Carroll et al. BMC Genomics 2012 13:561 doi:10.1186/1471-2164-13-561