Biological processes affected by inhibition of endogenous miR-181b in cell culture in response to anti-miR-181b transfection. Panel A illustrates the experimental design for the identification of genes subject to de-repression of PTGS by decreased endogenous miRNA concentrations. Genes elevated in response to a fall in miRNA were utilised for pathways analysis and correlated against predicted miRNA targets. Panel B shows the decrease in miR-181b expression levels in comparison to controls for HEK-293, HeLa and SH-SY5Y cell types. Panel C shows a clustered-by-gene heat map from whole genome expression microarray data from each cell model, with n=2 per condition. Panel D shows the significantly enriched KEGG pathways for each cell type in response to decreased intracellular miR-181b levels.
Carroll et al. BMC Genomics 2012 13:561 doi:10.1186/1471-2164-13-561