Open Access Research article

Oocyte-somatic cells interactions, lessons from evolution

Cathy Charlier1, Jérôme Montfort1, Olivier Chabrol5, Daphné Brisard2, Thaovi Nguyen1, Aurélie Le Cam1, Laurent Richard-Parpaillon4, François Moreews3, Pierre Pontarotti5, Svetlana Uzbekova2, Franck Chesnel4 and Julien Bobe1*

1 INRA, UR1037 LPGP Fish Physiology and Genomics, Campus de Beaulieu, Rennes, F-35000, France

2 INRA, UMR85 Physiologie de la Reproduction et des Comportements, CNRS 7247, Université de Tours, Nouzilly, F-37380, France

3 INRA, SIGENAE/PEGASE, Rennes, F-35000, France

4 CNRS, Université de Rennes 1, UMR6290, Rennes, F-35000, France

5 LATP UMR-CNRS, Aix-Marseille University 7353, Evolution Biologique et Modélisation, Marseille, F-13331, France

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BMC Genomics 2012, 13:560 doi:10.1186/1471-2164-13-560

Published: 19 October 2012

Additional files

Additional file 1:

Differentially expressed genes in trout, Xenopus, cow, and mouse. For each species, probe name and corresponding oligo sequence are shown for all differentially expressed spots. The corresponding normalized expression values are given for all studied samples. For each sample, the corresponding group -developmentally incompetent or poorly competent prophase I oocytes (NC1), developmentally competent prophase I oocytes (C1), or developmentally competent metaphase II oocytes (C2)- is indicated. Data from different species are displayed in separate datasheets. All corresponding data were submitted to Gene Expression Omnibus database under # GSE36617.

Format: XLSX Size: 4.6MB Download file

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Additional file 2:

RefSeq accession numbers used for the OrthoMCL analysis. For each species, the probe names and corresponding NCBI RefSeq accession numbers used for the OrthoMCL analysis are displayed. Data from different species are displayed in separate sheets of the file. For rainbow trout, the corresponding zebrafish protein accession numbers used in the analysis are shown.

Format: XLSX Size: 352KB Download file

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Additional file 3:

Orthologous trout and Xenopusgenes exhibiting a similar expression profile. For each OrthoMCL group of orthologous genes, corresponding RefSeq accession numbers and description are shown. Data from different species are displayed in separate datasheets.

Format: XLSX Size: 39KB Download file

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Additional file 4:

Pathways. DAVID-defined KEGG pathways annotation categories for the lists of genes similarly expressed in non-mammalian species (A), mammals (B), tetrapods (C) and in all four analysed species (D). For each set of common differential genes, list of all genes present on mice AgilentV2 microarray was used as a background. Analysis reported the score and p-value of enrichment of GO terms related to different pathways in each genes set. For differentially expressed genes common to Xenopus and trout (A), a zebrafish and a Xenopus laevis background were used.

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Additional file 5:

Orthologous mouse and cow genes exhibiting a similar expression profile. For each OrthoMCL group of orthologous genes, corresponding RefSeq accession numbers and description are shown. Data from different species are displayed in separate datasheets.

Format: XLSX Size: 150KB Download file

Open Data

Additional file 6:

Orthologous Xenopus, cow, and mouse genes exhibiting a similar expression profile. For each OrthoMCL group of orthologous genes, corresponding RefSeq accession numbers and description are shown. Data from different species are displayed in separate datasheets.

Format: XLSX Size: 72KB Download file

Open Data

Additional file 7:

Orthologous trout, Xenopus, mouse and cow genes exhibiting a similar expression profile. For each OrthoMCL group of orthologous genes, corresponding RefSeq accession numbers and description are shown. Data from different species are displayed in separate datasheets.

Format: XLS Size: 62KB Download file

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Additional file 8:

QPCR primers. The primer sequences are shown for all the genes analyzed using QPCR. For each OrthoMCL group, the accession numbers and primer sequences used in each species are shown.

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Additional file 9:

QPCR validation of specific gene expression profiles. Expression profiles of specific genes in somatic follicular cells during oocyte developmental competence acquisition: developmentally incompetent or poorly competent prophase I oocytes (NC1), developmentally competent prophase I oocytes (C1), and developmentally competent metaphase II oocytes (C2). Expression values were normalized using 18S in rainbow trout, Xenopus and mice, and using RPL19 in cow. Expression profiles obtained in the microarray analysis are shown. (M).

Format: PPTX Size: 88KB Download file

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