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Open Access Research article

Oocyte-somatic cells interactions, lessons from evolution

Cathy Charlier1, Jérôme Montfort1, Olivier Chabrol5, Daphné Brisard2, Thaovi Nguyen1, Aurélie Le Cam1, Laurent Richard-Parpaillon4, François Moreews3, Pierre Pontarotti5, Svetlana Uzbekova2, Franck Chesnel4 and Julien Bobe1*

Author Affiliations

1 INRA, UR1037 LPGP Fish Physiology and Genomics, Campus de Beaulieu, Rennes, F-35000, France

2 INRA, UMR85 Physiologie de la Reproduction et des Comportements, CNRS 7247, Université de Tours, Nouzilly, F-37380, France

3 INRA, SIGENAE/PEGASE, Rennes, F-35000, France

4 CNRS, Université de Rennes 1, UMR6290, Rennes, F-35000, France

5 LATP UMR-CNRS, Aix-Marseille University 7353, Evolution Biologique et Modélisation, Marseille, F-13331, France

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BMC Genomics 2012, 13:560  doi:10.1186/1471-2164-13-560

Published: 19 October 2012

Abstract

Background

Despite the known importance of somatic cells for oocyte developmental competence acquisition, the overall mechanisms underlying the acquisition of full developmental competence are far from being understood, especially in non-mammalian species. The present work aimed at identifying key molecular signals from somatic origin that would be shared by vertebrates.

Results

Using a parallel transcriptomic analysis in 4 vertebrate species - a teleost fish, an amphibian, and two mammals - at similar key steps of developmental competence acquisition, we identified a large number of species-specific differentially expressed genes and a surprisingly high number of orthologous genes exhibiting similar expression profiles in the 3 tetrapods and in the 4 vertebrates. Among the evolutionary conserved players participating in developmental competence acquisition are genes involved in key processes such as cellular energy metabolism, cell-to-cell communications, and meiosis control. In addition, we report many novel molecular actors from somatic origin that have never been studied in the vertebrate ovary. Interestingly, a significant number of these new players actively participate in Drosophila oogenesis.

Conclusions

Our study provides a comprehensive overview of evolutionary-conserved mechanisms from somatic origin participating in oocyte developmental competence acquisition in 4 vertebrates. Together our results indicate that despite major differences in ovarian follicular structure, some of the key players from somatic origin involved in oocyte developmental competence acquisition would be shared, not only by vertebrates, but also by metazoans. The conservation of these mechanisms during vertebrate evolution further emphasizes the important contribution of the somatic compartment to oocyte quality and paves the way for future investigations aiming at better understanding what makes a good egg.