Table 6

Primer sequences used to quantify expression of candidate genes from a third biological replicate using quantitative real-time PCR
Gene name Primer direction Sequence Accession number/ reference
Lipid Storage Droplet 2 Forward AATCGTGCTCG CAAAATACC XM_003249187.1
Reverse TTCGCAATAGGT AATGATTTTTCA
Bubblegum Forward TTTTGCAAATCT TGGTGCAA XM_624222.3
Reverse TTCTTCTGGTTT GCCTTCGT
Pale Forward TACTTCGTCGCG GATAGCTT NM_001011633.1
Reverse TACGCGAACGAT GTCTTCAG
Groucho Forward ACTGTGGACAGG AGGATTGG XM_392425.3
Reverse ATGAAGGACCTC GACGTTTG
Draper Forward ATCCTGCCACTG GTTATTGC XM_624852.2.
Reverse AGTCAACTCCCT CCCAACCT
Apterous Forward AGGAAGAGGAAA CCCAAGGA XM_392622.4
Reverse TCTGCGAGAGTTG CTTCAGA
Pebbled Forward CGCTGATACGTCA CCTCAGA XM_003249649.1
Reverse GCGAACTTCTCTC CACGTTC
Defensin 1 Forward TGCGCTGCTAACT GTCTCAG (Evans, [2006])
Reverse CGAAACGTTTGTC CCAGAG NM_001011616.2
Actin Forward CCTAGCACCATCC ACCATGAA (Huising & Flik, 2005)
Reverse GAAGCAAGAATTG ACCCACCAA

Primer sequences were designed in Primer3, v. 4.0 (http://frodo.wi.mit.edu/) or obtained from previous publications.

Richard et al.

Richard et al. BMC Genomics 2012 13:558   doi:10.1186/1471-2164-13-558

Open Data