Open Access Highly Accessed Research article

Effects of immunostimulation on social behavior, chemical communication and genome-wide gene expression in honey bee workers (Apis mellifera)

Freddie-Jeanne Richard13*, Holly L Holt2 and Christina M Grozinger23

Author Affiliations

1 Laboratoire Ecologie Evolution Symbiose, UMR CNRS 6556, University of Poitiers, 40 avenue du Recteur Pineau, Cedex, F-86022, POITIERS, France

2 Department of Entomology, Center for Pollinator Research, Center for Chemical Ecology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, 16802, USA

3 Previous address: Department of Entomology, North Carolina State University, Raleigh, NC, 27695, USA

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BMC Genomics 2012, 13:558  doi:10.1186/1471-2164-13-558

Published: 16 October 2012

Additional files

Additional file 1:

Table S1. All (670) significantly, differently expressed transcripts in Colony 1 (FDR<0.01). Table S2 All (1610) significantly, differently expressed transcripts in Colony 2 (FDR<0.01). Table S3 The 302 common, significantly, differently expressed transcripts in Colonies 1 and 2 (FDR<0.01) and their log-2 transformed, normalized, relative fold-expression values across treatment groups. Table S3b: p-values (FDR < 0.01) for all treatment pairwise comparisons (Control X Bacteria, Control X Bead, Control X Saline, Bacteria X Bead, Bacteria X Saline, Bead X Saline) for the 302 transcripts significantly regulated in both colonies (see Table S3a). Table S4 Common, significantly differentially expressed transcripts in treatment groups (control x bacteria, control x bead, control x saline) in both Colonies 1 and 2 (FDR < 0.01). Table S5 22 significantly, differentially regulated transcripts regulated by treatment (control x bacteria, control x bead, control x saline) in both Colonies 1 and 2 (FDR < 0.01). Table S6 Overrepresented GO categories (p < 0.05) amongst the 302 transcripts regulated by treatments in Colonies 1 and 2 (FDR < 0.01). Table S7 Overrepresented GO categories (p < 0.05) amongst the 22 transcripts regulated by all treatments in Colonies 1 and 2 (FDR < 0.01). Table S8 The 68 significantly, differently expressed transcripts in bacterial x control treatment groups in common between Colonies 1 and 2 (FDR<0.01). Table S9 Overrepresented GO categories (p < 0.05) amongst the 68 transcripts regulated by bacterial treatment only in Colonies 1 and 2 (FDR < 0.01). Table S10 14 genes that overlap between canonical immune genes (Evans et al., [2006]) and the 239 signficantly, differentially expressed genes in Colonies 1 and 2 (FDR < 0.01). Table S11 117 genes that were significantly regulated by Varroa infestation (Alaux et al, [2011]) and immune challenge (this study). Table S12 8 genes that were significantly regulated by immune challenge in honey bees (this study) and in Drosophila (De Gregario et al. [2011] and Roxstrom-Linquist et al. [2004]).

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