Table 5

Ratios of differentially expressed miRNAs in infarcted tissue using TaqMan based and Sybr Green approach
TaqMan Sybr Green
miR-1 miR-133a miR-133b miR-98 miR-21 miR-122 miR-125a-5p miR-125b miR-126
FFPE RNU48 0.10 1.24 0.25 0.36 nd nd nd nd nd
RNAlater RNU48 0.47 0.46 0.39 0.62 nd nd nd nd nd
FFPE RNU6B 0.11 0.68 0.29 0.41 0.59 1.71 0.49 0.44 0.29
RNAlater RNU6B 0.22 0.22 0.18 0.29 1.65 0.18 1.04 1.18 0.99
FFPE miR-26b 0.20 1.25 0.53 0.74 5.03 14.52 4.21 3.73 2.49
RNAlater miR-26b 0.78 0.77 0.64 1.02 2.64 0.29 1.67 1.90 1.59
microarray results 0.10 0.35 0.35 0.30 0.97 9.71 0.35 0.74 0.25

The ratios are calculated according to the efficiency corrected model [46]. Expression in remote myocardium is set at one, and expression in infarcted tissue is calculated as a proportion of the expression in corresponding remote myocardium. Further, three different genes were used as RGs. Legend: FFPE, formalin-fixed paraffin-embedded tissue; RNAlater, samples stored in RNAlater; RNU48, the small RNA RNU48 is used as RG; RNU6B, the small RNA RNU6B is used as RG; miR-26b, the miR-26b is used as RG.

Boštjančič et al.

Boštjančič et al. BMC Genomics 2012 13:552   doi:10.1186/1471-2164-13-552

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