Table 2

miRNAs with predicted influence on SERCA2a expression
MicroRNA ΔG binding (Kcal mol-1) ΔG 70 nt flanking 3’ of predicted binding site (Kcal mol-1) ΔG 70 nt flanking 5’ of predicted binding site (Kcal mol-1) Predicted binding position in 3’-UTR SERCA2a Perfect SERCA2a complimentarity to “seed” region
miR-122 −22.0 −14.9 −11.2 671-692 partially (1–6)
miR-199a-3p −32.1 −9.1 −14.1 76-97 no
−30.7 −11.1 −6.9 535-556 no
−24.3 −9.2 −6.9 705-726 no
−22.0 −7.6 −11.3 259-280 no
−20.5 −10.1 −6.6 26-47 no
miR-320a −24.6 −10.9 −7.1 117-138 partially (1–5, 7–12)
−21.7 −6.9 −11.1 536-557 partially (1–2, 4–8)
−24.8 −12.3 −11.4 505-526 partially (1–4, 7–8)
−25.5 −6.9 −12.9 229-250 no
−20.5 −8.5 −5.3 711-732 no
miR-320b −24.6 −10.9 −7.1 117-138 partially (1–5, 7–12)
−21.7 −6.9 −11.1 536-557 partially (1–2, 4–8)
−25.8 −12.3 −11.4 505-526 partially (1–4, 7–8)
−25.5 −6.9 −12.9 229-250 no
miR-320c −23.2 −6.9 −12.9 230-249 no
−23.3 −12.3 −11.4 507-526 partially (1–4, 7–8)
−19.5 −7.7 −5.3 713-732 no
miR-320d −24.4 −10.1 −7.1 120-138 partially (1–5, 7–13)
−19.4 −6.9 −11.1 539-557 partially (1–2, 4–8)
−22.8 −6.9 −12.9 231-249 no
−23.1 −12.3 −11.4 508-526 partially (1–4, 7–8)
−19.2 −6.4 −5.3 714-732 no
miR-483-5p −24.7 −9.8 −6.8 122-143 no
−24.1 −7.6 −6.3 708-729 no
miR-574-3p −27.8 −8.6 −5.3 725-746 yes
−32.5 −7.3 −9.7 37-58 partially (2–5, 7–13)
miR-574-5p −22.7 −7.3 −11.0 465-487 yes
−25.6 −14.5 −14.1 664-686 no
−30.2 −12.5 −0.3 740-762 no
−22.9 −8.0 −12.3 413-435 no
−21.6 −7.8 −11.1 364-386 no
miR-140-3p −19.1 −11.3 0.3 746-766 partially (1–4)
−20.2 −7.3 −9.7 38-58 yes
−24.2 −7.7 −5.3 713-733 yes

Boštjančič et al.

Boštjančič et al. BMC Genomics 2012 13:552   doi:10.1186/1471-2164-13-552

Open Data