Open Access Highly Accessed Research article

Identification of novel growth phase- and media-dependent small non-coding RNAs in Streptococcus pyogenes M49 using intergenic tiling arrays

Nadja Patenge1, André Billion2, Peter Raasch3, Jana Normann1, Aleksandra Wisniewska-Kucper1, Julia Retey4, Valesca Boisguérin5, Thomas Hartsch4, Torsten Hain2 and Bernd Kreikemeyer1*

Author Affiliations

1 Institute of Medical Microbiology and Hospital Hygiene, University of Rostock, Schillingallee 70, 18057, Rostock, Germany

2 Institute of Medical Microbiology, Genome Research, Justus-Liebig-University, Frankfurter Strasse 107, 35392, Giessen, Germany

3 Systems Biology and Bioinformatics Group, University of Rostock, 18051, Rostock, Germany

4 Genedata AG, Margarethenstrasse 38, 4053, Basel, Switzerland

5 febit biomed GmbH, Im Neuenheimer Feld 519, 69120, Heidelberg, Germany

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BMC Genomics 2012, 13:550  doi:10.1186/1471-2164-13-550

Published: 13 October 2012

Additional files

Additional file 1:

A: northern blot analysis of CRISPR gene expression and transcript processing in GAS M49; B: northern blot analysis of sRNASpy490957c gene expression in GAS M49.

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Additional file 2:

Sequences of primers used for 5 RACE.

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Additional file 3:

Sequences of gene-specific primers employed for qRT-PCR.

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