Table 2 |
||||||||
| Genome assembly statistics and summary | ||||||||
| Genome | Step | Software | Size a | # contigs b | # scaffolds | Average length c | N50 d | N90 e |
| Tcm | 454 assembly | CELERA | 37.3 | 30,737 | - | 1,216 | 1,670 | 539 |
| Illumina assembly | Velvet (kmer 43) | 16.7 | 9,247 | - | 1,813 | 2,378 | 851 | |
| Assembly of non-assembled Illumina reads | Velvet (kmer 53) | 1.17 | 2,094 | - | 562 | 536 | 418 | |
| Assembly merging | Zorro | 33.5 | 24,799 f | - | 1,353 | 2,218 | 549 | |
| Scaffolding | SSPACE | 38.8 | 23,813 f | 1,835 | 2,296 | 25,044 | 576 | |
| Gap closure | IMAGE | 38.6 | 23,000 | 1,774 | 2,302 | 25,781 | 583 | |
| Tcc X10 | 454 assembly | CELERA | 41.8 | 33,686 | - | 1,243 | 1,516 | 549 |
| Illumina assembly | Velvet (kmer 43) | 17.0 | 8,523 | - | 1,997 | 2,742 | 904 | |
| Assembly of non-assembled Illumina reads | Velvet (kmer 53) | 1.14 | 2,116 | - | 543 | 523 | 416 | |
| Assembly merging | Zorro | 38.0 | 28,389 f | - | 1,339 | 1,869 | 560 | |
| Scaffolding | SSPACE | 43.7 | 27,605 f | 2,476 | 2,162 | 14,067 | 589 | |
| Gap closure | IMAGE | 43.4 | 26,889 | 2,423 | 2,158 | 14,516 | 592 | |
a The length when sequences are combined (Mb).
b The number of contigs/scaffolds.
c The average contig length (bp). For the SSPACE row, this refers to the average scaffold length.
d The length N for which half of all bases are in a sequence of this length or longer.
e The length N for which 90% of all bases are in a sequence of this length or longer.
f Contigs >500 bp.
Franzén et al. BMC Genomics 2012 13:531 doi:10.1186/1471-2164-13-531