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Open Access Research article

Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)

Wanxing Wang1, Shunmou Huang2, Yumei Liu1*, Zhiyuan Fang1, Limei Yang1, Wei Hua2, Suxia Yuan1, Shengyi Liu2, Jifeng Sun1, Mu Zhuang1, Yangyong Zhang1 and Aisong Zeng1

Author affiliations

1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R.China. Beijing, 100081, China

2 Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Key Laboratory for Biological Sciences of Oil Crops, P.R.China. Ministry of Agriculture, Wuhan, 430062, China

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Citation and License

BMC Genomics 2012, 13:523  doi:10.1186/1471-2164-13-523

Published: 3 October 2012

Abstract

Background

Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a large number of SSR and SNP markers from the whole genome shotgun sequence data of B. oleracea, and the construction of a high-density genetic linkage map using a double haploid mapping population.

Results

The B. oleracea high-density genetic linkage map that was constructed includes 1,227 markers in nine linkage groups spanning a total of 1197.9 cM with an average of 0.98 cM between adjacent loci. There were 602 SSR markers and 625 SNP markers on the map. The chromosome with the highest number of markers (186) was C03, and the chromosome with smallest number of markers (99) was C09.

Conclusions

This first high-density map allowed the assembled scaffolds to be anchored to pseudochromosomes. The map also provides useful information for positional cloning, molecular breeding, and integration of information of genes and traits in B. oleracea. All the markers on the map will be transferable and could be used for the construction of other genetic maps.

Keywords:
Cabbage; Brassica; Genetic linkage map; SSR; SNP; Genome