Frequency distribution of unique RAD tag sequences from the parents of family HX13-WL before and after filtering and SNP discovery. Blue bars are the number of occurrences of each raw RAD sequence in both the male and female. The peak at the left is formed by sequences that only occur between one and five times and were removed from further analysis during filtering. Red bars show the distribution of the shared loci identified between the two parents (Table 1). Green shows the distribution of the 3,430 putative SNP containing loci from both parents. Note that the bars are not cumulative. The leftward shift in the polymorphic sequence distribution reflects that these loci were heterozygous in one or both parents and therefore have lower counts. There are scattered occurrences above a frequency count of 70, but the tail quickly asymptotes to near 0 with scattered single peaks.
Everett et al. BMC Genomics 2012 13:521 doi:10.1186/1471-2164-13-521