Testing duck scaffolds aligning to two chicken chromosomes. Based on previous observations, duck scaffolds aligning to two chicken chromosomes were suspected to be misassembled and one example is shown here. A: sca3008, boxed in red, aligns to GGA5 and GGA7, according to the Narcisse database. B: Markers sca3008A (green) and sca3008B (purple), very close to one another on sca3008, but spanning the putative breakpoint, were genotyped on the RH panel, but failed to show linkage, indicating that the scaffold is indeed misassembled. Results for other scaffolds are shown in Additional File 3: Figure S2.
Rao et al. BMC Genomics 2012 13:513 doi:10.1186/1471-2164-13-513