Confirmation of the inversion on APL12 by FISH. Chromosomes are stained by DAPI. Centromere positions (cen) are indicated by arrows. Left: BAC clone WAG19G7, corresponding to duck scaffold sca2558 is located in the centromeric region of GGA11 (top), whereas it is clearly located in the middle of the q arm of APL12 (bottom), suggesting the occurrence of an intrachromosomal rearrangement. Right: BAC clone WAG19G7 (red) corresponds to scaffold2558, whereas WAG20C21 and WAG13P2 (green) to scaffold1176. In chicken WAG19G7 (scaffold2558) is located in the centromeric region of GGA11 and WAG20C21 (scaffold1176) is in the middle of the q arm (top), whereas in duck, WAG19G7 (scaffold2558) is located in the middle of the q arm and WAG13P2 (scaffold1176) is at the end (bottom). This suggests the occurrence of an inversion between the two species. The black bands in the middle of APL12 near BAC clone 19 G7, might be an artifact resulting from over-denaturation or to the DAPI staining.
Rao et al. BMC Genomics 2012 13:513 doi:10.1186/1471-2164-13-513