Experimental Workflow and Study Design. This Figure shows the tested samples (individual, pooled, non-amplified gDNA, and matched WGA) using the established T-NGS workflow (for more details see Additional file 3: Table S1). Each enriched individual sample was barcoded and sequenced under the following conditions: 1) one sample per octet, 3) samples were pooled after SOLiD library construction, pre-emulsion PCR (emPCR), and 3) samples were pooled post-emPCR. To assess the reproducibility of the established T-NGS method, equimolar amounts of the enriched six HapMap samples were also pooled before SOLiD library construction and tested in duplicate (Libraries ID 768_1L and 768_2L are technical replicates).
ElSharawy et al. BMC Genomics 2012 13:500 doi:10.1186/1471-2164-13-500