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Open Access Research article

Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters

David D O’Keefe1, Sean R Thomas2, Kelsey Bolin4, Ellen Griggs4, Bruce A Edgar13 and Laura A Buttitta4*

Author affiliations

1 Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA

2 Gladstone Institute for Cardiovascular Disease, J David Gladstone Institutes, San Francisco, CA, 94158, USA

3 German Cancer Research Center (DKFZ)-Center for Molecular Biology Heidelberg (ZMBH) Alliance, Im Neuenheimer Feld 282, Heidelberg, D-69120, Germany

4 Molecular Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA

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Citation and License

BMC Genomics 2012, 13:498  doi:10.1186/1471-2164-13-498

Published: 20 September 2012

Abstract

Background

The transformation of a developing epithelium into an adult structure is a complex process, which often involves coordinated changes in cell proliferation, metabolism, adhesion, and shape. To identify genetic mechanisms that control epithelial differentiation, we analyzed the temporal patterns of gene expression during metamorphosis of the Drosophila wing.

Results

We found that a striking number of genes, approximately 50% of the Drosophila transcriptome, exhibited changes in expression during a time course of wing development. While cis-acting enhancer sequences clearly correlated with these changes, a stronger correlation was discovered between core-promoter types and the dynamic patterns of gene expression within this differentiating tissue. In support of the hypothesis that core-promoter type influences the dynamics of expression, expression levels of several TATA-box binding protein associated factors (TAFs) and other core promoter-associated components changed during this developmental time course, and a testes-specific TAF (tTAF) played a critical role in timing cellular differentiation within the wing.

Conclusions

Our results suggest that the combinatorial control of gene expression via cis-acting enhancer sequences and core-promoter types, determine the complex changes in gene expression that drive morphogenesis and terminal differentiation of the Drosophila wing epithelium.

Keywords:
Drosophila melanogaster; Core promoter; Cis-regulatory sequence; Microarray; Metamorphosis; Wing Morphogenesis; Terminal differentiation; Cell cycle exit; TATA box binding protein-associated factor