Validation of data from mRNA-Seq. (A, B) Graphical representation of the correlation between modENCODE expression data of E12-14hs embryos and the mRNA-Seq expression data for WT16 (A) and E18-20hs embryos with the mRNA-Seq expression data for WT17 (B). Correlation coefficients are 0.79776 and 0.81202, respectively. (C, D) Graphical representation of the expression of dro5 (C) and IM3 (D) obtained by Q-PCR in the indicated genotypes. Ho: homozygous sall mutant embryos. U (upregulated) and E (equal) refer to the expression analysis by mRNA-Seq of these genes at the indicated genotypes’ comparisons.
Ferreiro et al. BMC Genomics 2012 13:483 doi:10.1186/1471-2164-13-483