Additional file 2.

Screening of origin activity inH. hispanica. A. Schematic of the ARS assay. Δ: Corresponding origin (or cdc6 plus intergenic region)-deletion H. hispanica strains (unpublished data) were used for transformation to avoid plasmid integration. * For the two origins, oriC1-cdc6A and oriP-cdc6K, which cannot be knocked out from the chromosome and megaplasmid, respectively, the wide-type strains were used for transformation and Southern blot was performed to confirm ARS activity (Figure  1). B. ARS assay plates for eleven candidates. Colonies in plates of AS-168 (Mev) were observed after 7 days at 37 °C, and the minus signs (−) represent no visible colonies (no ARS activity).

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Wu et al. BMC Genomics 2012 13:478   doi:10.1186/1471-2164-13-478