Table 5

Primers and PCR conditions for generation of the digoxigenin-labeled probes
Primer Sequence Probe PCR conditions
Hem_8081_FW 5'-CAATATGACTACCGACCCGGTTAC-3' Hem_8081 - Denaturation at 95°C for 2 min
Hem_8081_RV 5'-GGATACATCTGCTGGGCGATATAC-3' 162 bp - 30 cycles:
denaturation at 95°C for 30 sec
Col_WA_FW 5'-CGATCGTAGTAGTAAGGCAACTCC-3' Col_WA annealing at 60°C for 30 sec
Col_WA_RV 5'-GACGGTATCATGCCCATAACTG-3' 234 bp elongation at 72°C for 40 sec
- Final elongation at 72°C for 7 min

Garzetti et al.

Garzetti et al. BMC Genomics 2012 13:467   doi:10.1186/1471-2164-13-467

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