Ligase treatment drastically reduces PCR efficiency in TUF regions. The charts indicate the amplicon product ratios for two PRT assays for four ‘old’ DNA samples (C7, C8, D4, D5) that were untreated (blue) and treated with PreCR (red), which includes a ligase to repair ssDNA nicks. Treated samples have greatly reduced amplification efficiency at the test amplicon in the ‘TUF’ region compared to the untreated DNA samples.
Veal et al. BMC Genomics 2012 13:455 doi:10.1186/1471-2164-13-455