Table 5

Correlation between differences in carbon source utilisation and genotype of B. pilosicoli 95/1000, B2904 and WesB
Unique carbon source compound tested 95/1000 B2904 WesB Possible explanation
D-Mannose - - + WesB is the only strain with the mannose/sorbose-specific PTS system IIABCD components (wesB_1269, wesB_1270, wesB_1271 and wesB_1272) for uptake and phosphorylation of D-mannose.
D-Glucuronic acid - + + 95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links D-glucuronic acid metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf900) and WesB (wesB_1781).
D-Mannitol - + - B2904 is the only strain with the D-mannitol PTS system IIABC components (B2904_orf2447) and also a mannitol-1-phosphate 5-dehydrogenase (B2904_orf2446) for D-mannitol, uptake, phosphorylation and catabolism.
Glucuronamide - + + 95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links glucuronate and related compound metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf899 and B2904_orf900) and WesB (wesB_1781).
β-D-Allose - - + WesB is the only strains with D-allose ABC transporter components (wesB_1171, wesB_1172 and wesB_1175) and D-allose kinase (wesB_0259 and wesB_1174) for uptake and metabolism of D-allose.
β-Methyl-D-glucuronic acid - + + 95/1000 lacks the pfkB carbohydrate kinase, 2-dehydro-3-deoxygluconate kinase, which links glucuronate and related compound metabolism to glycolysis. This enzyme is found in both B2904 (B2904_orf899 and B2904_orf900) and WesB (wesB_1781).
L-Sorbose - - + WesB is the only strain with the mannose/sorbose-specific PTS system IIABCD (wesB_1269, wesB_1270, wesB_1271, wesB_1272) components for uptake and phosphorylation of L-sorbose.

Possible explanations for the differences in phenotype relate to differences in genomic features.

Mappley et al.

Mappley et al. BMC Genomics 2012 13:454   doi:10.1186/1471-2164-13-454

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