Open Access Research article

Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity

Eun-Young Kwon1, Su-Kyung Shin1, Yun-Young Cho1, Un Ju Jung1, Eunjung Kim2, Taesun Park3, Jung Han Yoon Park4, Jong Won Yun5, Robin A McGregor1, Yong Bok Park6 and Myung-Sook Choi17*

Author Affiliations

1 Center for Food and Nutritional Genomics Research, Kyungpook National University, Daegu, Republic of Korea

2 Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongsan, Republic of Korea

3 Department of Food and Nutrition, Yonsei University, Seoul, Republic of Korea

4 Department of Food Science and Nutrition, College of Natural Sciences, Hallym University, Chuncheon, Republic of Korea

5 Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea

6 School of Life Science & Biotechnology, Kyungpook National University, Daegu, Republic of Korea

7 Department of Food Science and Nutrition, Kyungpook National University, 1370 Sank-Yuk Dong Puk-Ku, Daegu, 702-701, Korea

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BMC Genomics 2012, 13:450  doi:10.1186/1471-2164-13-450

Published: 4 September 2012

Additional files

Additional file 1:

Table S1.Composition of experimental diets. 1AIN-76 mineral mixture (grams/kg): calcium phosphate 500, sodium chloride 74, potassium citrate 2220, potassium sulfate 52, magnesium oxide 24, magnesium carbonate 3.5, ferric citrate 6, zinc carbonate 1.6, cupric carbonate 0.3, potassium iodate 0.01, sodium celenite 0.01, chromium potassium sulfate 0.55, sucrose 118.03, 2AIN-76 vitamin mixture (grams/kg): thiamin HCL 0.6, riboflavin 0.6, pyridoxin HCL 0.7, niacin 3, calcium pantothenate 1.6, folic acid 0.2, biotin 0.02, vit B12 1, vit A(500,000U/gm) 0.8, vit D3(400,000U/gm) 0.25, vit E acetate(500 U/gm) 10, menadione sodium bisulfite 0.08, sucrose 981.15, ND: normal diet (AIN-76), HFD: high-fat diet (20% fat, 1% cholesterol)

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Additional file 2:

Table S2.Primer sequences used for RT-qPCR validation of microarray data.

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Additional file 3:

Table S3.Body weight gain, food intake and food efficiency in C57BL/6 J mice fed the different diets for 24 weeks. Data shown as means ± S.D. Values are significantly different from the ND group according to Student's t-test: *p < 0.05, **p < 0.01, ***p < 0.001. ND: normal diet (AIN-76), HFD: high-fat diet (20% fat, 1% cholesterol), FER: food efficiency ratio.

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Additional file 4:

Table S4.List of high-fat diet responding gene transcripts in epididymal WAT that simultaneously associated with advancing age in ND and HFD groups for 24 weeks.

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