Genetic map of the sigH-rshA operon, its Northern hybridization analysis in C. glutamicum RES167 and its deletion derivatives. A. Genetic map of the sigH-rshA region showing locations and sizes of deletions in the chromosomes of strains C. glutamicum ΔrshA, DN2 and AS1, predicted sizes of respective sigH-rshA and rshA transcripts (arrows) and locations of probes used for Northern hybridizations. Promoters are indicated with bent arrows and the terminator with a hairpin symbol. B. Northern blot using a sigH probe (left panel) and an rshA probe (right panel) hybridized with total RNA extracted from: RES167 cells (lane 1); DN2 cells (ΔsigH deletion; lane 2); AS1 cells (ΔsigHrshA deletion; lane 3). The estimated lenghts of the detected transcripts (left) and their designations (right) are indicated. The sizes of the fragments in the RNA marker are indicated with arrows.
Busche et al. BMC Genomics 2012 13:445 doi:10.1186/1471-2164-13-445