Open Access Research article

Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum

Tobias Busche1, Radoslav Šilar2, Martina Pičmanová2, Miroslav Pátek2 and Jörn Kalinowski1*

Author Affiliations

1 Centrum für Biotechnologie, Universität Bielefeld, 33594, Bielefeld, Germany

2 Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská, 1083, Prague 4, Czech Republic

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BMC Genomics 2012, 13:445  doi:10.1186/1471-2164-13-445

Published: 3 September 2012

Additional files

Additional file 1:

Amino acid sequence alignment between the three corynebacterial genes and their M. tuberculosis and S. coelicolor counterparts. Alignment of RshA from C. glutamicum, C. efficiens, C. diphtheriae and C. jeikeium, as well as M. tuberculosis and RsrA of Streptomyces coelicolor by CLUSTALX [60] Conserved cysteines are boxed. Identical residues are indicated with an asterisk, ":" indicates a stronger degree of conservation, and "." indicates a weaker degree of conservation.

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Additional file 2:

Genes differentially transcribed in C. glutamicum ΔrshA compared to C. glutamicum RES167 (reference) sorted by ratio. A comparative microarray hybridization analyses was performed using total RNA isolated from C. glutamicum RES167 [47] and its rshA deletion derivative growing under standard cultivation conditions (30°C) in shaking flasks. The differentially transcribed genes are listed in this table. Altogether, 83 genes in 61 putative transcriptional units were found to be upregulated in the ΔrshA mutant compared to its parental strain and 38 genes were downregulated, only 7 exceeded the chosen threshold m < −1 (fold change 2/0.5). Microsoft Excel.

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Additional file 3:

Primers used in this work. Microsoft Word.

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