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Open Access Research article

Small interfering RNA-producing loci in the ancient parasitic eukaryote Trypanosoma brucei

Christian Tschudi1, Huafang Shi2, Joseph B Franklin3 and Elisabetta Ullu23*

Author Affiliations

1 Department of Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, 06536, USA

2 Department of Internal Medicine, School of Medicine, Yale University, New Haven, CT, 06536, USA

3 Department of Cell Biology, School of Medicine, Yale University, New Haven, CT, 06536, USA

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BMC Genomics 2012, 13:427  doi:10.1186/1471-2164-13-427

Published: 27 August 2012

Additional files

Additional files 1:

Summary of total and unique reads.

Format: XLSX Size: 11KB Download file

Open Data

Additional files 2:

Total and unique siRNAs at two pseudogenes in wild-type (wt),dcl1−/− and dcl2−/− cells. siRNAs were normalized to the total number of reads (setlength).

Format: XLSX Size: 13KB Download file

Open Data

Additional files 3:

Total small RNAs at each convergent transcription units (CTUs) in wild-type (wt), dcl1−/− and dcl2−/− cells. siRNAs were normalized to the total number of reads (setlength).

Format: XLSX Size: 23KB Download file

Open Data

Additional files 4:

Sequences of oligonucleotides used for preparing probes for hybridization.

Format: DOCX Size: 14KB Download file

Open Data

Additional files 5:

Total small RNAs at various regions of the genome with no clear features. Listed as "miscalleneous" in Table 2.

Format: XLSX Size: 14KB Download file

Open Data