CcpA expression levels in B. subtilis cultures used for DNA microarray experiments. (A) upper panel, CcpA detection using anti-CcpA antibody; lower panel, signal quantification with ImageJ. Four CcpA expression levels were achieved by growing B. subtilis strain MP902 (Ptet-ccpA, Pxyl-tetR) in absence (lanes 1, 3 and 5) and in presence of 0.1, 2 and 20 nM ATc (lanes 2, 4 and 6), respectively. All cultures were grown in presence of 0.2 % xylose and 1 % glucose. Shadows in the background of the picture indicate culture pairs used in microarray experiments (B) Ponceau S control membrane staining for protein load verification. Lane numbers correspond to lane numbers in panel A. The representative graphs of three reproducible experiments are shown.
Marciniak et al. BMC Genomics 2012 13:401 doi:10.1186/1471-2164-13-401