Table 1

Amplification, sequencing and site-directed mutagenesis primers for RNase 8

Gene-amplification

RN8F

5'- CAC ATT GCC CTG CAA TAA CTG GCT -3'

RN8R

5'- AGG TTG AGT GTG TGG GAG GGA AAT -3'

Sequencing

RNase 8 Fi

5'- TCA CTC TGT TCC ACT GTC TCC CTT -3'

RNase 8 Ri

5'- AGT CTG CAG AGT GTG AG GTG GAA -3'

Human RNase 8 nested RACE (human spleen cDNA)

rcRN8 (3' → 5')

5'-CAA CTT TAT CCA AGT GCA CAG GA-3'

R85Rn (3' → 5')

5'-CTG GGT CAC CCT GTT GTG GAG-3'

Human RNase 8 nested reverse-trancriptase PCR (RT-PCR)

R8S

5'-ACA ATA GAA TGC CAG GGG TGT TCA-3'

R85R

5'-CAA CTT TAT CCA AGT GCA CAG GA-3'

R8Sn

5'-CAT TGC CCT GCA ATA ACT GGC TT-3'

R85Rn

5'-CTG GGT CAC CCT GTT GTG GAG-3'

Human RNase 8 quantitative RT-PCR

RNase 8fx

5'- CAT AGC CTG CAA GAA TAG CTG TAA AA-3'

RNase 8rx

5'- TCA CCC ATG GTC AGG GAC AT-3'

6FAM-RNase 8-TAMRA

6FAM-CTG CCA CCA GAG CCA CGG GC-TAMRA

Nested primate genome RNase 8

5'R8F

5'-GCC AGG GGT GTT CAA TAT CTT AG-3'

3'R8R

5'-AGG TTG AGT GTG TGG GAG GGA AAT-3'

5'R8Fn

5'-CAC ATT GCC CTG CAA TAA CTG GCT-3'

3'R8Fn

5'-AGT CTG CAG AGT GTG AGG TGG AAC-3'

RNase 8 expression plasmids

CCC3

R8D

5'-AAG CTT ATT GCC CTG CAA TAA CTG GCT TAG GGT-3'

R8C

5'-CTC CTC CTC TCT AGA GAC AAC TTT ATC CAA GTG CAC A-3'

CCC4

R8O

5'-AAG CTT ACT CTG TTC CAC TGT CTC CCT TAA GAG AG-3'

R8C

5'-CTC CTC CTC TCT AGA GAC AAC TTT ATC CAA GTG CAC A-3'

CCC5 mutagenesis

R85F

5'-TCT CCC TTA AGA GAG ATA GCA CCG GCC AGA GCA-3'

R85R

5'-TGC TCT GGC CGG TGC TAT CTC TCT TAA GGG AGA-3'


Chan et al. BMC Genomics 2012 13:40   doi:10.1186/1471-2164-13-40

Open Data