PCR verification of the overlapping relationship between pairs of overlapping BAC clones. Pairs of overlapped BAC clones were PCR amplified using a primer pair designed based on the BAC-end sequence. The markers above the black lines define the primer pairs and the ones below the lines are numbers of positive clones used as PCR templates.
Li et al. BMC Genomics 2012 13:398 doi:10.1186/1471-2164-13-398