Open Access Highly Accessed Research article

Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray

Wen-Ming Qiu, An-Dan Zhu, Yao Wang, Li-Jun Chai, Xiao-Xia Ge, Xiu-Xin Deng and Wen-Wu Guo*

Author Affiliations

Key Laboratory of Horticultural Plant Biology (Ministry of Education); National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

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BMC Genomics 2012, 13:397  doi:10.1186/1471-2164-13-397

Published: 16 August 2012

Additional files

Additional file 1:

Figure S1. Comparison of expression patterns of 4 ESTs assembled the some contig encoding Methionine synthase. Y-axis represents the average Cy5 (Cy3) to Cy3 (Cy5) ratio in array hybridization. X-axis represents the four developmental stages.

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Additional file 2:

Figure S2. The correlation of gene expression ratios between cDNA microarray and qRT-PCR. Data were from 11 probe sets at four developmental stages. The gene expression ratios based on cDNA microarray were in log2 transformed.

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Additional file 3:

Sequences shorter than 200 bp but longer than 100 bp.

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Additional file 4:

Figure S3. The purified PCR products for microarray probe. 100 bp molecular ladders were used.

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Additional file 5:

Table S1. qRT-PCR primers for 11 candidate genes and citrus actin gene.

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Additional file 6:

Table S2. qRT-PCR primers for 10 transcription factors (TFs).

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