Figure 4.

Methylation profile and antisense transcript analysis for the novel DMR identified in intron 11 of M. domestica IGF2R.(A) IGF2R is represented graphically. The three sets of primers used in strand-specific RT-PCR analysis are labeled as 1, 2 and 3. The reverse transcription primers for anti-sense strand detection are labeled as 1a, 2a, and 3a while those for sense-strand detection are labeled as 1b, 2b, and 3b. (B) Parent-of-origin analysis was performed at the DMR by analyzing methylation of cloned alleles for an individual bearing an (A/T) SNP at chr2: 442,443,695 with a mother homozygous for A at the same location. Only the allele inherited from the mother is methylated. (C) Strand-specific RT-PCR analysis using primers described above shows a PCR product only by reverse transcription utilizing 1b, 2b band 3b; L represents the 100 bp DNA ladder. This demonstrates that the gene is expressed at this locus but there is no associated anti-sense transcript.

Das et al. BMC Genomics 2012 13:394   doi:10.1186/1471-2164-13-394
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