Analysis of imprinting at the MEST locus in M. domestica. (A) 3′ RACE distinguished two alternative MEST transcripts. The vertical arrows indicate the polymorphic sites used to analyze the imprint status of the two transcripts. (B) Transcript 1 (n = 2) is imprinted since the genomic A/G polymorphism is absent in the cDNA, demonstrating that only one allele is expressed. The parental origin of expression for transcript 1 could not be determined because a heterozygous offspring with a mother homozygous at the A/G polymorphic site was not found. In contrast, transcript 2 (n = 1) is not imprinted since the C/A polymorphism is present in both the genomic DNA and the cDNA.
Das et al. BMC Genomics 2012 13:394 doi:10.1186/1471-2164-13-394