Additional file 5.
Figure S2. Amplification curves for the ihfB gene in different strains. This figure shows the positions of the amplification curves for the ihfB gene (Section A) and the Ct values of this gene (Section B), in the different strains employed in this study. As can be seen, all the amplification curves of the ihfB gene that has been used as the reference gene for the determination of the RT-qPCR levels, have very similar values. The values presented in the table are from three different fermentations (F1, F2 y F3) for each utilized strain. Since all the values included in section B are very similar, only one third of them are presented (labeled with an asterisk *) in section A. These results demonstrate that the same reproducible expression levels were obtained for the ihfB gene in all strains. This is the most important characteristic that a reference gene should have, in agreement to the MIQE guidelines [59-61]. These results corroborate the stability of the expression of the reference ihfB gene in these strains in the utilized conditions. (EPS 308 kb)
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Aguilar et al. BMC Genomics 2012 13:385 doi:10.1186/1471-2164-13-385