Phylogenetic tree. (A) Phylogenetic relationships among 21 fungi (including M. brunnea, S. sclerotiorum, M. grisea, N. crassa, Fusarium graminearum, Aspergillus fumigatus, Coccidioides immitis, Stagonospora nodorum, Candida albicans, Debaryomyces hansenii, Candida glabrata, Saccharomyces cerevisiae, Kluyveromyces lactis, Yarrowia lipolytica, Schizosaccharomyces pombe, Coprinopsis cinereus, U. maydis, Puccinia graminis f. sp. tritici, Rhizopus oryzae, Batrachochytrium dendrobatidis, and Monosiga brevicollis), Caenorhabditis brenneri, and Marssonina coronariae. To show the evolutionary relationships of the 23 species, a phylogenetic tree was constructed using the concatenated amino acid sequences with 1000 bootstraps. There were five main clusters, including Ascomycota fungi, Bssidiomycetes fungi and Nematoda, Mucoromycotina fungi, Chytridiomycosis fungi, and Monosiga brevicollis as outgroup. (B) The ITS sequences of Marssonina coronariae (MC, four strains), Marssonina brunnea (MB, Marssonina brunnea f. sp. multigermtubi), Marssonina rosae (MR, four strains), Botrytis cinerea (BC, two strains) and Sclerotinia sclerotiorum (SS, two strains) were used for constructing the phylogenetic tree. ITS sequences were aligned using ClustalW (v 2.1). A Neighbor-joining tree was built with 1000 bootstraps by MEGA (v4.0.2). The GenBank accession No. of the ITS sequences used for phylogenetic tree analysis are shown in Table S6.
Zhu et al. BMC Genomics 2012 13:382 doi:10.1186/1471-2164-13-382