The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses
1 Department of Molecular Biology, Microbiology and Biochemistry, Southern Illinois University at Carbondale, Carbondale, IL 62901, USA
2 Department of Plant Soil and Agricultural Systems, Southern Illinois University at Carbondale, Carbondale, IL 62901-4415, USA
3 Department of Horticulture and Crop Science, Ohio State University, 2021 Coffey Rd, Columbus, OH 43210, USA
4 Agriculture and Agri-Food Canada, Building 21, 960 Carling Ave, Ottawa, ON K1A 0C6, USA
5 Key Laboratory of Soybean Biology in the Chinese Ministry of Education, Harbin University, Harbin, China
6 JCVI, Rockville, MD, USA
7 Department of Plant Breeding & Genetics, Rajasthan College of Agriculture, MPUAT, Udaipur, India
8 USDA, Crop Genetics Research Unit, Jackson, TN, USA
9 Genomics Core Facility; Center for Excellence the Illinois Soybean Center, Southern Illinois University at Carbondale, Carbondale, IL 62901-4415, USA
BMC Genomics 2012, 13:368 doi:10.1186/1471-2164-13-368Published: 2 August 2012
Additional file 1::
Figure S1. Paralogs of Rhg1 in the soybean genome. Panel (A) shows LRR probe (200bp) hybridized to Forrest MTP. (B) Southern hybridization of LRR probe (200bp) to the MTP positives. Five out of the 7 MTP clones hybridized after BAC clone purification and restriction digestion with HindIII. The lower panel (C) shows the kinase domain probe (200bp) hybridized to Forrest MTP. Panel (D) shows Southern hybridization of the same kinase probe to the MTP positives. Three out of 5 MTP clones hybridized after BAC clone purification and restriction digestion with HindIII. Panel F shows ideograms of the genes predicted from the genome sequences centered on GmRLK18-1 and GmRLK11-1 Panel F shows an alignment of the genome sequences of 70kbp centered on GmRLK18-1 and GmRLK11-1 showing the extent of synteny.
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Additional file 2::
Table S1. Comparisons of sequence identity between Forrest alleles of GmRLK18-1 and GmRLK11-1 the most similar and syntenic RLK like protein. The amino acid identity was 78% in the signal peptide (residues 1–61); 94% in the ten LRRs (141–471), 93% in the transmembrane domain (485–507) and 97% in the kinase domain (569–840). Residues that differ in alloproteins of GmRLK18-1 are in bold. Four of the six are identical in the homeoprotein the other 2 are identical to the susceptible allele. The neighboring laccase and the antiporter also showed 85–96% amino acid identity.
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Additional file 3::
Figure S2. Detection of the SNP polymorphism at position 1486 in the LRR region of Rhg1 –a and –e using an allelic discriminatory assay. A Famlabeled probe was used for the detection of resistant haplotypes1 and 2 (red) and Hex labeled probe for the detection of susceptible haplotypes2, 3 and 4 (blue). A total of 16 individuals from the 110 PIs were selected for the analysis. The Panel shows relative fluorescent signal intensity for each of the 16 plant introductions. The two groups form separate clusters.
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