Table 1

An. gambiaeRNA-seq Expression Data
Infected Non-polysomal Infected Polysomal Uninfected Non-polysomal Uninfected Polysomal Uninfected Total RNA Infected Total RNA
Total Reads Set 1 7785361 4244042 4781123 4766035 2081115 6150051
Total Reads Set 2 3871849 3442387 3351084 2203276 9837495 4328298
Total Reads Set 3 6597022 6980908 8588835 5980648 3009585 8407264
Reads Mapped to Transcriptome Set 1 3527074 (45.3%) 3423900 (80.7%) 3608010 (75.5%) 1049208 (22.0%) 1483831 (71.3%) 5144550 (83.7%)
Reads Mapped to Transcriptome Set 2 2332634 (60.2%) 1476806 (42.9%) 2557909 (76.3%) 473146 (21.5%) 7772228 (79.0%) 2612416 (60.4%)
Reads Mapped to Transcriptome Set 3 4942339 (74.9%) 5191081 (74.4%) 7221237 (84.1%) 4992710 (83.5%) 2634845 (71.3%) 5992586 (71.3%)

Sequence reads are listed for three independent experiments (Sets 1–3). The six samples were multiplexed and sequenced together. Each set had roughly 30 million raw reads. Erroneous reads and reads where linkers were incomplete or missing were excluded from total number of reads. Reads mapped to transcriptome are the number (and percentage) of reads which matched back to the An. gambiae cDNA P3.4 database.

Mead et al.

Mead et al. BMC Genomics 2012 13:366   doi:10.1186/1471-2164-13-366

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