Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Open Badges Research article

Translational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection

Edward A Mead12, Meng Li1, Zhijian Tu1 and Jinsong Zhu1*

Author Affiliations

1 Department of Biochemistry, Virginia Tech, 311 Engel Hall, Blacksburg, VA, 24061, USA

2 Current address: Department of Animal Sciences, Rutgers University, New Brunswick, NJ, 08901, USA

For all author emails, please log on.

BMC Genomics 2012, 13:366  doi:10.1186/1471-2164-13-366

Published: 2 August 2012

Additional files

Additional file 1:

Table S1.Analysis of the steady-state mRNA levels and mRNA association with polysomes during Plasmodium infection. (XLSX 470 kb)

Format: XLSX Size: 471KB Download file

Open Data

Additional file 3:

Figure S1.Plasmodium falciparum oocyst counts in Anopheles gambiae at 7–9 days post blood feeding. Midguts were examined in 56 female mosquitoes that had been fed on P. falciparum-infected blood. Each “*” represents one mosquito. 64.3% of the mosquitoes were infected. It is possible that the actual infection rate was higher as we possibly did not remove away some of the unfed mosquitoes. The infection rate represents a conservative estimate. The average infected midgut with oocysts had 2.22 oocysts. The image on the right panel shows oocysts that we observed in a representative mosquito. (JPEG 985 kb)

Format: JPEG Size: 986KB Download file

Open Data

Additional file 2:

Table S2.Primers used in qRT-PCR.

Format: DOCX Size: 11KB Download file

Open Data