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Open Access Highly Accessed Research article

Translational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection

Edward A Mead12, Meng Li1, Zhijian Tu1 and Jinsong Zhu1*

Author Affiliations

1 Department of Biochemistry, Virginia Tech, 311 Engel Hall, Blacksburg, VA, 24061, USA

2 Current address: Department of Animal Sciences, Rutgers University, New Brunswick, NJ, 08901, USA

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BMC Genomics 2012, 13:366  doi:10.1186/1471-2164-13-366

Published: 2 August 2012

Additional files

Additional file 1:

Table S1.Analysis of the steady-state mRNA levels and mRNA association with polysomes during Plasmodium infection. (XLSX 470 kb)

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Additional file 3:

Figure S1.Plasmodium falciparum oocyst counts in Anopheles gambiae at 7–9 days post blood feeding. Midguts were examined in 56 female mosquitoes that had been fed on P. falciparum-infected blood. Each “*” represents one mosquito. 64.3% of the mosquitoes were infected. It is possible that the actual infection rate was higher as we possibly did not remove away some of the unfed mosquitoes. The infection rate represents a conservative estimate. The average infected midgut with oocysts had 2.22 oocysts. The image on the right panel shows oocysts that we observed in a representative mosquito. (JPEG 985 kb)

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Additional file 2:

Table S2.Primers used in qRT-PCR.

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