Table 1

Samples used in the TaqMan Copy Number Assay for theGreylocus
ID Grey Type2 Breed Comments
Calibrator g/g C_DNA Arabian Calibrator in analysis
Ref G/g C_DNA Thoroughbred Genotype reference
Ref G/G C_DNA Lipizzaner Genotype reference
SP1 G/g1 C_DNA Connemara Late greying phenotype
SP2 G/g1 C_DNA Connemara Late greying phenotype
SP3 G/g1 C_DNA Connemara Late greying phenotype
Ho-Mel-L1 G/g Equine melanoma cell line Lipizzaner Normal growth
Ho-Mel-A1 G/g Equine melanoma cell line Arabian Fast growth
M1 G/g Equine melanoma cell line Irish warmblood Normal growth
M14 G/G Equine melanoma cell line Andalusian Fast growth
ID1 G/g tumour DNA Thoroughbred Euthanized due to melanoma tumours
ID2 G/G C_DNA + tumour DNA Shagya Arabian Euthanized due to melanoma tumours
ID3 G/g C_DNA + tumour DNA Swedish warmblood Euthanized due to melanoma tumours
ID4 G/g C_DNA + tumour DNA Connemara Cremello coloured3, euthanized due to laminitis, very small melanomas

1 Not genotyped with Grey long-range PCR assay due to fragmented DNA; inferred genotype from copy number assay.

2 C_DNA = constitutional genomic DNA from blood or hair samples.

3 Cremello horses are homozygous for a missense mutation D153N in SLC45A2 (MATP) and show a severe dilution of pigmentation in hair, skin and eye [25].

Sundström et al.

Sundström et al. BMC Genomics 2012 13:365   doi:10.1186/1471-2164-13-365

Open Data