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Open Access Highly Accessed Research article

Characterization of microRNAs expression during maize seed development

Mingming Kang, Qian Zhao, Dengyun Zhu and Jingjuan Yu*

Author Affiliations

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

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BMC Genomics 2012, 13:360  doi:10.1186/1471-2164-13-360

Published: 1 August 2012

Additional files

Additional file 1:

Conserved maize miRNAs expression level. The total reads of conserved miRNAs and corresponding miRNA*s, sorted by miRNA names. Expression difference were shown in column 4.

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Additional file 2:

Expression of conserved miRNAs validated by miRNA microarray. The detailed normalized signals in the microarray experiment, including whole probes.

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Additional file 3:

Secondary structure of novel miRNA precursors. Predicted secondary structures of newly identified miRNAs, mature miRNA sequences were shown in red, while the miRNA* sequences were shown in blue, if any.

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Open Data

Additional file 4:

Novel maize miRNAs identified in this study. Detailed information of newly identified miRNAs.

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Additional file 5:

Genome locations of novel miRNA precursors. The genome locations of novel miRNA precursors based on the current annotation of maize genome (5b.60).

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Additional file 6:

Potential targets of novel miRNAs. The filtered set of targets that had no mismatch in position 2-12 and no consecutive mismatch, filtered from the original set that passed the criteria described above. Annotations were retrieved from the InterPro database.

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Additional file 7:

Primers used in this study.

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