|Gene set enrichment analysis (GSEA) of classifiers on their original gene expression profiles (GEPs) for training|
|Chemical||Brain GEP (F-ratio)||Brain classifiers enriched on brain GEP (FDR)||Ovary GEP (F-ratio)||Ovary classifiers enriched on ovary GEP (FDR)|
|Fadrozole (FAD)||FAD-brain (3.41)||No enrichment||FAD-ovary (2.01)a||PRO-ovary (0.005)|
|17α-ethynyl estradiol (EE2)||EE2-brain (5.96)||EE2-brain (0.028)||EE2-ovary (22.4)b||KET-ovary (0.002)|
|Muscimol (MUS)||MUS-brain (6.42)||MUS-brain (0.0)||MUS-ovary (42.62)b||FIP-ovary (0.027)|
|Fipronil (FIP)||FIP-brain (6.78)||FIP-brain (0.002); MUS-brain (0.012)||FIP-ovary (10.23)||FIP-ovary (0.006);|
|Flutamide (FLU)||NA||FLU-ovary (67.23)||KET-ovary (0.002)|
|Ketoconazole (KET)||KET-brain (9.17)||
|KET-ovary (4.85)||KET-ovary (0.0)|
|Prochloraz (PRO)||PRO-brain (19.42)||PRO-brain (0.0)||PRO-ovary (14.48)||PRO-ovary (0.0)|
|17β-trenbolone (TRE)||TRE-brain (22.94)b||No enrichment||TRE-ovary (3.28)a||KET-ovary (0.1)|
|Trilostane (TRI)||NA||TRI-ovary (11.16)b||PRO-ovary (0.121)|
|Vinclozolin (VIN)||NA||VIN-ovary (6.09)||VIN-ovary (0.024);|
|F-ratio mean = 10.59||F-ratio mean = 18.44|
Each classifier was formatted into a gene set, and multiple gene sets were grouped by their tissue of origin. GSEA was conducted by individual chemical-tissue conditions, at a threshold of FDR ≤ 0.25. Classifiers enriched on the top or bottom of a ranked gene list are separated by a semicolon. If two classifiers of the same condition are both significant, only the most significant one is listed. A GEP in bold indicates the availability of its corresponding classifier as a gene set. FDR, false discovery rate; F-ratio, maximum Fisher’s discriminant ratio; “NA“, a condition not included in the study.
aSearched but no qualified classifier found for the condition. Its GSEA was conducted using classifiers for other unrelated chemical-tissues.
bNo classifier search was conducted due to insufficient sample size for the condition. Its GSEA was conducted using classifiers for other unrelated chemical-tissues.
Wang et al.
Wang et al. BMC Genomics 2012 13:358 doi:10.1186/1471-2164-13-358