ChIP-qPCR data suggest that RosR binds DNA directly. Relative enrichment ratio is shown for a 100-bp region in the sod2 promoter region in immunoprecipitates (IP) compared to randomly sheared chromosomal DNA (whole cell extract, WCE). Enrichments are compared for the putative VNG0258H transcription factor, empty plasmid (“mock”) and TrmB (a transcription factor known not to bind to the sod2 locus ). Error bars represent +/− SEM from the mean of 4 biological replicate experiments. (B) sod2 (superoxide dismutase) is overexpressed in ΔrosR vs. the parent strain in H2O2 gene expression experiments. Red trace represents microarray gene expression data (also shown in Figure 5A) for sod2 in ΔrosR, whereas the black trace is for the Δura3 parent strain. (C) sod2 (superoxide dismutase) is overexpressed in ΔrosR vs the parent strain in PQ gene expression experiments. Colors are as in (B).
Sharma et al. BMC Genomics 2012 13:351 doi:10.1186/1471-2164-13-351