Additional file 1.

Construction plasmids and confirmation of a reference strain and a strain only expressing the hacA induced form. Schematic representation of the plasmids pHAC (A) and pConstHac (B) (Note: fragment sizes are not on scale). (C) Sequence alignment of pHAC and pConstHAC showing the absence of the 20 nt intron on pConstHac. (D) PCR amplification of gDNA of HacAWT (NC1.1) and HacACA (NC2.1) transformants. Primers were designed about 100 bp upstream and 100 bp downstream of the hacA intron region, giving rise to a band of 200 bp for HacACA and 220 bp for HacAWT. Sizes of the DNA Marker (M) are indicated.

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Carvalho et al. BMC Genomics 2012 13:350   doi:10.1186/1471-2164-13-350