Table 2

Correlation between microarray and qRT-PCRresults
Gene locus Gene name Function of gene product Change fold (824ccpA/824WT)
Middle exponential phase (M) Stationary phase (S)
Microarray qRT-PCR* Microarray qRT-PCR*
CAC1345 xylT L-arabinose-proton symporter 71.6 92.52 ± 2.55 147.39 326.54 ± 176.86
CAC2612 xylB Xylulose kinase 26.9 20.4 ± 4.00 17.9 21.21 ± 0.02
CAC1348 tkt1 Transketolase 5.6 4.42 ± 0.23 7.9 6.4 ± 0.21
CAC1941 abrB1941 Stationary/sporulation gene regulator 5.1 89.17 ± 7.31 1.62 1.62 ± 0.30
CAC1660 bukII Butyrate kinase II 4.3 7.03 ± 2.02 5.2 10.79 ± 0.04
CAC3319 - Signal transduction histidine kinase 3.95 2.7 ± 1.01 3.43 2.41 ± 0.30
CAC2966 lacR Lactose phosphotransferase system repressor 1.95 6.88 ± 1.43 1.13 15.49 ± 5.61
CAC3647 abrB3647 Stationary/sporulation gene regulator 0.67 0.47 ± 0.05 0.48 0.61 ± 0.11
CAC1653 - Cell wall biosynthesis glycosyltransferase 0.43 0.34 ± 0.10 0.56 0.41 ± 0.11
CAP0164 ctfB Butyrate-acetoacetate COA-transferase subunit B 0.27 0.17 ± 0.01 1.00§ 0.24 ± 0.06
CAC0103 cysC Adenylylsulfate kinase 0.25 0.04 ± 0.01 182 49.14 ± 41.18
CAC0570 glcG PTS enzyme II, ABC component 0.16 0.05 ± 0.02 0.19 0.34 ± 0.14
CAC0310 abrB310 Stationary/sporulation gene regulator 0.14 0.62 ± 0.08 0.18 0.59 ± 0.22
CAC1341 araD L-ribulose-5-phosphate 4-epimerase 2.29 2.11 ± 0.50 5.81 2.33 ± 1.80
CAC1343 ptk Phosphoketolase 1.12 1.84 ± 0.59 2.87 2.35 ± 0.71

The wild-type (824WT) and ccpA-inactivated mutant strain (824ccpA) were cultured in P2 medium using 40 g/L D-glucose and 20 g/L D-xylose as the carbon sources. Cells were harvested at middle exponential phase (A600 ≈ 1.0) and early stationary phase (A600 ≈ 12.0).

* The data were mean ± standard deviation (SD) of two independent biological replicates.

§ The microarray result of the ctfB gene at S phase was inaccurate because its expression level exceeded the detection upper limit.

Ren et al.

Ren et al. BMC Genomics 2012 13:349   doi:10.1186/1471-2164-13-349

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