Table 1 |
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| Technical specifications of Next Generation Sequencing platforms utilised in this study | |||||
| Platform | Illumina MiSeq | Ion Torrent PGM | PacBio RS | Illumina GAIIx | Illumina HiSeq 2000 |
| Instrument Cost* | $128 K | $80 K** | $695 K | $256 K | $654 K |
| Sequence yield per run | 1.5-2Gb | 20-50 Mb on 314 chip, 100-200 Mb on 316 chip, 1Gb on 318 chip | 100 Mb | 30Gb | 600Gb |
| Sequencing cost per Gb* | $502 | $1000 (318 chip) | $2000 | $148 | $41 |
| Run Time | 27 hours*** | 2 hours | 2 hours | 10 days | 11 days |
| Reported Accuracy | Mostly > Q30 | Mostly Q20 | <Q10 | Mostly > Q30 | Mostly > Q30 |
| Observed Raw Error Rate | 0.80 % | 1.71 % | 12.86 % | 0.76 % | 0.26 % |
| Read length | up to 150 bases | ~200 bases | Average 1500 bases**** (C1 chemistry) | up to 150 bases | up to 150 bases |
| Paired reads | Yes | Yes | No | Yes | Yes |
| Insert size | up to 700 bases | up to 250 bases | up to 10 kb | up to 700 bases | up to 700 bases |
| Typical DNA requirements | 50-1000 ng | 100-1000 ng | ~1 μg | 50-1000 ng | 50-1000 ng |
* All cost calculations are based on list price quotations obtained from the manufacturer and assume expected sequence yield stated.
** System price including PGM, server, OneTouch and OneTouch ES.
*** Includes two hours of cluster generation.
**** Mean mapped read length includes adapter and reverse strand sequences. Subread lengths, i.e. the individual stretches of sequence originating from the sequenced fragment, are significantly shorter.
Quail et al. BMC Genomics 2012 13:341 doi:10.1186/1471-2164-13-341
